| 紫杉醇微悬臂梁免疫传感器方法的研究 |
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| 引用本文： | 薛长国,赵洪伟,吴尚犬,南铁贵,王保民,张青川,伍小平.紫杉醇微悬臂梁免疫传感器方法的研究[J].分析化学,2011,39(6):863-866. |
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| 作者姓名： | 薛长国 赵洪伟 吴尚犬 南铁贵 王保民 张青川 伍小平 |
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| 作者单位： | 薛长国,吴尚犬,张青川,伍小平,XUE Chang-Guo,WU Shang-Quan,ZHANG Qing-Chuan,WU Xiao-Ping(中国科学技术大学,中国科学院材料力学行为和设计重点实验室,合肥,230027);赵洪伟,南铁贵,王保民,ZHAO Hong-Wei,NAN Tie-Gui,WANG Bao-Min(中国农业大学农学与生物技术学院,北京,100094) |
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| 基金项目： | 国家自然科学基金，教育部科技创新工程重大项目培育资金(No.708053)资助课题 |
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| 摘 要： | 以巯基化的紫杉醇单抗修饰微梁镀金表面,制备了高灵敏的紫杉醇微悬臂梁免疫传感器.利用酶联免疫吸附测定方法对巯基化前后紫杉醇单抗的活性变化,以及巯基化单抗在微梁上的修饰进行检测与验证.采用紫杉醇微悬臂梁免疫传感器对不同浓度的紫杉醇溶液进行检测.结果表明:虽然巯基化后紫杉醇单抗的活性降低了18.6%,但巯基化紫杉醇单抗可以修...
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| 关 键 词： | 微悬臂梁免疫传感器 紫杉醇 单克隆抗体 |
Research on Paclitaxel Microcantilever Immunosensor |
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| XUE Chang-Guo,ZHAO Hong-Wei,WU Shang-Quan,NAN Tie-Gui,WANG Bao-Min,ZHANG Qing-Chuan,WU Xiao-Ping.Research on Paclitaxel Microcantilever Immunosensor[J].Chinese Journal of Analytical Chemistry,2011,39(6):863-866. |
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| Authors: | XUE Chang-Guo ZHAO Hong-Wei WU Shang-Quan NAN Tie-Gui WANG Bao-Min ZHANG Qing-Chuan WU Xiao-Ping |
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| Institution: | XUE Chang-Guo1,ZHAO Hong-Wei2,WU Shang-Quan1,NAN Tie-Gui2,WANG Bao-Min*2,ZHANG Qing-Chuan*1,WU Xiao-Ping1 1(Key Laboratory of Mechanical Behavior and Design of Material of Chinese Academy of Sciences,University of Science and Technology of China,Hefei 230027) 2(College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094) |
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| Abstract: | A highly sensitive paclitaxel microcantilever immunosensor was fabricated using sulfhydrated antipaclitaxel monoclonal antibody immobilized on the gold surface of the microcantilever.An enzyme-linked immunosorbent assay was used to evaluate the activity changes of anti-paclitaxel monoclonal antibody before and after sulfhydrylation,and the bingding activity of sulfhydrated anti-paclitaxel monoclonal antibody immobilized on the gold surface of the microcantilever.The experimental results indicated that altho... |
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| Keywords: | Microcantilever immunosensor Paclitaxel Monoclonal antibody |
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