| 毛细管电泳法分离四环素及其杂质 |
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| 引用本文： | 李永民,陈立仁,俞惟乐,van Schepdael A.,Roets E.,Hoogmartens J..毛细管电泳法分离四环素及其杂质[J].分析测试技术与仪器,1996(3):1-7. |
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| 作者姓名： | 李永民 陈立仁 俞惟乐 van Schepdael A. Roets E. Hoogmartens J. |
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| 作者单位： | 中国科学院兰州化学物理研究所 |
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| 摘 要： | 用毛细管电泳方法分离四环素和它的主要杂质４－差向四环素，脱水四环素，４－差向脱水四环素和２－乙酰基－２－脱酰胺四环素，相对次要的杂质氯四环素和脱甲四环素也能被分离。并系统研究了缓冲液ｐＨ，缓冲液浓度以及毛细管温度和电压对分离的影响。紫外检测波长是２７０ｎｍ，测定的相对标准偏差为１．９％。所得结果与ＨＰＬＣ方法进行了比较
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| 关 键 词： | 四环素 相关杂质 毛细管 电泳分离测定 纯度控制 |
| 收稿时间： | 5/8/1996 12:00:00 AM |
| 修稿时间： | 5/8/1996 12:00:00 AM |
Purity Control of Tetracycline with Capillary Electrophoresis |
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| Li Yongmin,Chen Liren,Yu Weile,van Schepdael A.,Roets E. and Hoogmartens J..Purity Control of Tetracycline with Capillary Electrophoresis[J].Analysis and Testing Technology and Instruments,1996(3):1-7. |
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| Authors: | Li Yongmin Chen Liren Yu Weile van Schepdael A Roets E and Hoogmartens J |
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| Institution: | Lanzhou Institute of Chemical Physics, Chinese Academy of sciences, Lanzhou 730000;Lanzhou Institute of Chemical Physics, Chinese Academy of sciences, Lanzhou 730000;Lanzhou Institute of Chemical Physics, Chinese Academy of sciences, Lanzhou 730000;Katholieke Universiteit Leuven, B-3000, Leuven, Belgium;Katholieke Universiteit Leuven, B-3000, Leuven, Belgium;Katholieke Universiteit Leuven, B-3000, Leuven, Belgium |
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| Abstract: | A method for purity control of tetracycline with capillary electrophoresis is described.It allows the complete separation of tetracycline and its main impurities 4-epitetracycline,anhydrotetracycline,4-epianhydrotetracycline and 2-acety-2-decarboxamidotetracycline.Less important chlortetracycline and demethyltetracycline can also be resolved.Method development was undertaken with a mixture consisting of tetracycline and its main impurities mentioned.Using an uncoated fused silica capillary,the infiuences of buffer pH,buffer concentration,temperature and applied voltage were systematically investigated.UV-detection was performed at 270 nm.A relative standard deviation of 1.9% was obtained for tetracycline.The results are compared with those of liquid chromatography and existing capillary electrophoresis. |
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| Keywords: | tetracycline impurities capillary electrophoresis purity control assay |
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