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A Synthetic Substrate of DNA Polymerase Deviating from the Bases,Sugar, and Leaving Group of Canonical Deoxynucleoside Triphosphates
Authors:Mi-Yeon Jang  Xiao-Ping Song  Mathy Froeyen  Philippe Marlière  Eveline Lescrinier  Jef Rozenski  Piet Herdewijn
Institution:1. Rega Institute for Medical Research, Katholieke Universiteit Leuven, Leuven, 3000, Belgium;2. Institute of Systems and Synthetic Biology, University of Evry-Val-d’Essonne, Evry, F-91030, France
Abstract:
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  • Highlights? A highly modified nucleotide as substrate for polymerases ? The reversibility of the polymerase reaction at the template level ? Synthesis of a nucleoside with two anomeric centers
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