Application of factorial design to the optimization of peroxidase activity in reverse micelles of bis(2-ethylhexyl)sodium sulfosuccinate/isooctane |
| |
| Authors: | Ana Morna-Gomes Maria Luisa Serralheiro Pedro Salema Fevereiro Maria Raquel Aires-Barros |
| |
| Affiliation: | (1) Centro de Engenharia Biológica e Química, Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Av. Rovisco Pais, 1000 Lisboa, Portugal;(2) Departamento de Química e Bioquímica, Centro de Ciência e Tecnologia de Materiais, Faculdade de Ciências de Lisboa da Universidade de Lisboa, 1700 Lisboa, Portugal;(3) Laboratório de Biotecnologia de Plantas, ITQB, Quinta do Marquês, 2780 Oeiras, Portugal |
| |
| Abstract: | ![]()
Two cationic peroxidases isolated from Vaccinium myrtillus were encapsulated in reverse micelles of bis(2-ethylhexyl)sodium sulfosuccinate/isooctane. By using a central composite design, some relevant parameters for the enzymatic activity, such as surfactant and water concentration, pH, and buffer molarity, were analyzed. With the results obtained from this experimental planning, the response surface curves were established. The maximum specific activity obtained (0.19 mM/min · mM of enzyme) was approximately the same for both peroxidases, but the experimental conditions under which this value was attained differed considerably. |
| |
| Keywords: | Peroxidase reverse micelles activity factorial design response surface |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
