| 微孔板化学发光法检测碱性磷酸酶技术的建立 |
| |
| 引用本文： | 刘欢,胡亚萍,高习习,王鹏,丁凡,王钦富.微孔板化学发光法检测碱性磷酸酶技术的建立[J].分析科学学报,2017,33(2). |
| |
| 作者姓名： | 刘欢 胡亚萍 高习习 王鹏 丁凡 王钦富 |
| |
| 作者单位： | 大连大学生命科学与技术学院,辽宁大连,116622 |
| |
| 摘 要： | 碱性磷酸酶(ALP)能催化发光底物APLS迅速水解并持续发光,据此建立了化学发光技术检测ALP活性的方法。利用一种新的发光底物APLS,建立微孔板化学发光法检测ALP活性,并对实验条件进行优化。优化后的最适反应条件为:微孔板上每孔加200μL APLS,在pH=9.5、37℃条件下与ALP反应10min,多功能酶标仪检测发光光子数(RLU),检测ALP线性范围是0.01~1U/L。微孔板化学发光法检测ALP,敏感度更高,且操作简便易行。
|
| 关 键 词： | 碱性磷酸酶 化学发光法 微孔板 |
Microplate Chemiluminescence Method for Alkaline Phosphatase Detection |
| |
| LIU Huan,HU Ya-ping,GAO Xi-xi,WANG Peng,DING Fan,WANG Qin-fu.Microplate Chemiluminescence Method for Alkaline Phosphatase Detection[J].Journal of Analytical Science,2017,33(2). |
| |
| Authors: | LIU Huan HU Ya-ping GAO Xi-xi WANG Peng DING Fan WANG Qin-fu |
| |
| Abstract: | Alkaline phosphatase(ALP) can catalyze its substrate,leading to emit-ting light continuously.In this paper,a new substrate,APLS,was used,and a chemiluminescence technique for detecting alkaline phosphatase on microplate was developed and optimized.The results showed that the linear range for the determination of ALP was 0.01-1 U/L.Compared with other techniques for detecting ALP,this method is more sensitive and simpler. |
| |
| Keywords: | Alkaline Phosphatase Chemiluminescence technique Microplate |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
