凝胶色谱净化超高效液相色谱法测定食品中10种工业染料

建立了测定食品中10种工业染料的超高效液相色谱(UPLC)法.样品经提取后,采用凝胶色谱(GPC)净化后收集浓缩,以乙腈-0.1％甲酸溶液为流动相进行梯度洗脱,10种染料得到良好分离.10种染料线性关系良好,相关系数R2＞0.9992,其检出限为0.011～0.049 μg /mL;样品在低、中、高3个加标浓度下,10种染料的回收率为65.0％～107.2％.结果表明该方法适用于食品中10种工业染料的检测,其样品处理方法适用于各类食品,且检测灵敏度高,可实现食品中工业染料的快速测定.

Simultaneous Determination of Synthetic Dyes in Foods by Ultra Performance Liquid Chromatography Coupled with Gel Permeation Chromatography Clean-up Procedure

A simultaneous detection method of 10 synthetic dyes in foods was developed using ultra performance liquid chromatography.After extraction,the samples were purified by gel permeation chromatography and then concentrated.The sample solution was separated in gradient elution with acetonitrile-0.1％ formic acid solution as the mobile phase.The linear relationships were good with the correlation coefficient R2 ＞0.9992.Detection limits were 0.011-0.049 μg/mL.The average recoveries for 10 dyes were 65.0％-107.2％.The results indicated that the developed method was rapid and sensitive in determination of synthetic dyes.