Improving FAIMS sensitivity using a planar geometry with slit interfaces |
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Authors: | Ridha Mabrouki Ryan T Kelly David C Prior Alexandre A Shvartsburg Keqi Tang Richard D Smith |
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Institution: | aBiological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington, USA |
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Abstract: | Differential mobility spectrometry or field asymmetric waveform ion mobility spectrometry (FAIMS) is gaining broad acceptance
for analyses of gas-phase ions, especially in conjunction with largely orthogonal separation methods such as mass spectrometry
(MS) and/or conventional (drift tube) ion mobility spectrometry. In FAIMS, ions are filtered while passing through a gap between
two electrodes that may have planar or curved (in particular, cylindrical) geometry. Despite substantial inherent advantages
of the planar configuration and its near-universal adoption in current stand-alone FAIMS devices, commercial FAIMS/MS systems
have employed curved FAIMS geometries that can be more effectively interfaced to MS. Here we report a new planar (p-) FAIMS
design with slit-shaped entrance and exit apertures that substantially increase ion transmission in and out of the analyzer.
The entrance slit interface effectively couples p-FAIMS to multi-emitter electrospray ionization (ESI) sources, improving
greatly the ion current introduced to the device and allowing liquid flow rates up to ~50 μL/min. The exit slit interface
increases the transmission of ribbon-shaped ion beams output by the p-FAIMS to downstream stages such as a MS. Overall, the
ion signal in ESI/FAIMS/MS analyses increases by over an order of magnitude without affecting FAIMS resolution. |
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