Preparation of a piezoelectric immunosensor for the detection of Salmonella paratyphi A by immobilization of antibodies on electropolymerized films |
| |
| Authors: | Shihui Si Fenglian Ren Wen Cheng Shouzhuo Yao |
| |
| Institution: | (1) Department of Chemistry, Central South University of Technology, Changsha 410083, P.R. China, CN;(2) New Material Research Institute, Human University, Changsha, P.R. China, CN |
| |
| Abstract: | To obtain new coatings for the preparation of piezoelectric immunosensors, the anodic polymerization of o-aminophenol (oAP),
o-phenylenediamine (oPD) and m-phenylenediamine (mPD) onto a gold-plated crystal has been studied. The possibility of immobilizing
an antibody (anti-S. paratyphi A) onto the crystal via the electropolymerized films was investigated. The poly-mPD film gave
the best results for immobilizing the antibody. With antibodies crosslinked on the poly-mPD film, a piezoelectric immuno-assay
for the detection of S. paratyphi A was proposed. The shift (ΔF=F20s−F400s, Hz) between the frequency at 20 s after the addition of sample (F20s), and that of 400 s (F400s) was used to construct a calibration graph, and shortening of the assay time was achieved. The S. paratyphi A concentration
in the range of 105–109 cells/ml can be measured by this method.
Received: 9 May 1996/Revised: 6 September 1996/Accepted: 19 September 1996 |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
