Uptake of magnetic nanoparticles into cells for cell tracking |
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| Authors: | Christiane Becker,Michael Hodenius,Gitta Blendinger,Antonio Sechi,Thomas Hieronymus,Detlef Mü ller-Schulte,Thomas Schmitz-Rode,Martin Zenke |
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| Affiliation: | 1. Department of Cell Biology, Institute for Biomedical Engineering, Aachen University Medical School, Pauwelsstrasse 30, Aachen 52074, Germany;2. Department of Applied Medical Engineering, Institute for Biomedical Engineering, Aachen University Medical School, Rheinisch-Westfälische Technische Hochschule (RWTH), 52074 Aachen, Germany;3. Helmholtz Institute for Biomedical Engineering, Rheinisch-Westfälische Technische Hochschule (RWTH), 52074 Aachen, Germany;4. MagnaMedics, BioPartner Center, Maastricht, The Netherlands |
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| Abstract: | ![]()
A challenge for future applications in nanotechnology is the functional integration of nano-sized materials into cellular structures. Here we investigated superparamagnetic Fe3O4 iron oxide nanoparticles coated with a lipid bilayer for uptake into cells and for targeting subcellular compartments. It was found that magnetic nanoparticles (MNPs) are effectively taken up into cells and make cells acquire magnetic activity. Biotin-conjugated MNPs were further functionalized by binding of the fluorescent tag streptavidin–fluorescein isothiocyanate (FITC) and, following uptake into cells, shown to confer magnetic activity and fluorescence labeling. Such FITC-MNPs were localized in the lysosomal compartment of cells which suggests a receptor-mediated uptake mechanism. |
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| Keywords: | Magnetic nanoparticle MNP Iron oxide particle Magnetoliposome Cell tracking |
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