The DI-SPME Method for Determination of Selected Narcotics and Their Metabolites,and Application to Bone Marrow and Whole Blood Analysis |
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| Authors: | Magdalena Ś wią dro-Pię toń ,Alicja Chromiec,Marcin Zawadzki,Renata Wietecha-Posł uszny |
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| Affiliation: | 1.Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 2 Gronostajowa St., 30-387 Kraków, Poland; (M.Ś.-P.); (A.C.);2.Department of Forensic Medicine, Medical University in Wroclaw, 4 Jana Mikulicza-Radeckiego St., 50-345 Wrocław, Poland; |
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| Abstract: | The present investigation utilised the quick and easy SPME/LC-MS method to determine selected narcotic substances and their metabolites in whole blood. The study included qualitative analysis and validation of the method. Analytes were determined in the linearity range of 25–300 ng/mL. The precision during and between days (in general CV < 13.41%), and the LOD which results in between 0.36 and 11.08 ng/mL, and the LOQ between 1.20 and 36.90 ng/mL were investigated. The validation results obtained, as well as the results of subsequent in-laboratory tests, confirmed the applicability of the method in the analysis of blood samples. An attempt to apply the method to the analysis of bone marrow samples has yielded promising results; however, more detailed studies are needed in this area. |
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| Keywords: | bone morrow narcotics substances SPME method LC-MS technique |
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