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High-throughput bioscreening system utilizing high-performance affinity magnetic carriers exhibiting minimal non-specific protein binding |
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| Authors: | Naohiro Hanyu Mamoru Hatakeyama Toshiyuki Tanaka Masaru Tada Adarsh Sandhu Masanori Abe Hiroshi Handa |
| Institution: | a Tokyo Bio R & D Center, Motortronics Laboratory, Tamagawa Seiki Co. Ltd., 4259-3 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, Japan b Department of Biological Information, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, Japan c Integration Research Institute, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo, Japan d Department of Physical Electronics, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo, Japan e Quantum Nanoelectronics Research Center, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro-ku, Tokyo, Japan |
| Abstract: | For affinity purification of drug target protein we have developed magnetic carriers, narrow in size distribution (184±9 nm), which exhibit minimal non-specific binding of unwanted proteins. The carriers were highly dispersed in aqueous solutions and highly resistant to organic solvents, which enabled immobilization of various hydrophobic chemicals as probes on the carrier surfaces. Utilizing the carriers we have automated the process of separation and purification of the target proteins that had been done by manual operation previously. |
| Keywords: | Magnetic carrier Bioscreening Affinity purification Target protein Automated screening system |
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