A radioanalytical technique for measurement of beta-glucuronidase activities期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

A radioanalytical technique for measurement of beta-glucuronidase activities

Authors:	T. Ünak U. Avcibaşi Y. Yildirim

Affiliation:	(1) Ege University, Faculty of Science, Department of Chemistry, Division of Nuclear Chemistry, Bornova, Izmir 35100, Turkey;(2) Ege University, Faculty of Science, Department of Chemistry, Division of Nuclear Chemistry, Bornova, Izmir 35100, Turkey;(3) Ege University, Faculty of Science, Department of Chemistry, Division of Nuclear Chemistry, Bornova, Izmir 35100, Turkey

Abstract:	Summary Phenolphthalein-glucuronide is a commonly used glucuronide conjugate for beta-glucuronidase measurements. The quantity of phenolphthalein liberated by beta-glucuronidase is measured spectrophotometrically. The detection limit of the quantity of phenolphthalein using spectrophotometry is a few μg. In this study, a new radioanalytical technique for the measurement of beta-glucuronidase was applied which is 10⁶times more sensitive than the spectrophotometric technique. Radioiodinated phenolphthalein-glucuronide and phenyl-N-glucuronide were used in this study in which the beta-glucuronidase levels of some tissue samples were measured.

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