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| 用傅里叶变换红外光谱研究增强UV-B辐射对PSⅡ蛋白结构的影响 | |
| 引用本文： | Liu X,Tang WT,Li Q,Yue M. 用傅里叶变换红外光谱研究增强UV-B辐射对PSⅡ蛋白结构的影响[J]. 光谱学与光谱分析, 2011, 31(1): 65-68. DOI: 10.3964/j.issn.1000-0593(2011)01-0065-04 |
| 作者姓名： | Liu X Tang WT Li Q Yue M |
| 作者单位： | 西北大学,西部资源生物与现代生物技术教育部重点实验室,陕西西安,710069 |
| 基金项目： | 国家自然科学基金项目(30670366); 陕西省自然科学基金项目(2009JQ3004)资助 |
| 摘 要： | 运用傅里叶变换红外光谱技术研究了增强紫外线-B(UV-B)辐射对植物光系统Ⅱ(PSⅡ)中膜蛋白结构的影响.结果显示在增强的UV-B辐射条件下,PSⅡ中蛋白的α-螺旋、β-折叠强度增加,而β-转角的强度下降了近一倍,并且这些结构与周围的耦合减弱.此外,UV-B辐射使酪氨酸残基苯酚环的结构发生改变,并使其微极性降低.UV-... |
| 关 键 词： | UV-B 红外光谱 PSⅡ 蛋白结构 |
Effects of ultraviolet-B radiation on protein structure in PS II using Fourier transform infrared spectroscopy |
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| Liu Xiao,Tang Weng-Ting,Li Qian,Yue Ming. Effects of ultraviolet-B radiation on protein structure in PS II using Fourier transform infrared spectroscopy[J]. Spectroscopy and Spectral Analysis, 2011, 31(1): 65-68. DOI: 10.3964/j.issn.1000-0593(2011)01-0065-04 | |
| Authors: | Liu Xiao Tang Weng-Ting Li Qian Yue Ming |
| Affiliation: | LIU Xiao,TANG Weng-ting,LI Qian,YUE Ming* Key Laboratory of Resource Biology and Biotechnology in Western China,Ministry of Education,Northwest University,Xi'an 710069,China |
| Abstract: | The effects of ultraviolet-B (UV-B) radiation on protein structure in photosystem II (PS II) was studied using Fourier transform infrared spectroscopy. The results demonstrated that irradiation of the plant with enhanced UV-B caused the change in the structures of protein in PSII. The intensity of alpha-helix, beta-sheet was increased, while the intensity of beta-turn was decreased. The coupling of those structures with surrounding became weaker with UV-B radiation. Otherwise, the UV-B influenced the structure of tyrosine residue and made the polarity of tyrosine decrease. The changes caused by UV-B radiation could alter the combination between Mn cluster and pigments with protein inevitably. It would lead to a series of changes, such as the process of light transmission and conversion, electron transfer and oxygen evolution. |
| Keywords: | UV-B |
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