C型肉毒毒蛋白的拉曼光谱研究

Ｃ型肉毒毒蛋白的拉曼光谱研究伍铁桥，刘新民，黄天荃，鲍培谛，杨志荣，刘世贵（四川大学成都６１００６４）ＳｔｕｄｉｅｓｏｎＴｈｅＲａｍａｎＳｐｅｃｔｒｏｓｃｏｐｙｏｆＢｏｔｕｌｉｎＴｙｐｅＣ￥ＷｕＴｉｅｑｉａｏ；ＬｉｕＸｉｎｍｉｎ；ＨｕａｎｇＴｉａｎｑ...

Studies on The Raman Spectroscopy of Botulin Type C

Abstract This paper reports the results of Raman spectroscopy of botulin type C. The results show that natural protein and renatured protein have dif- ferent space structure. The natured protein （BC_1）has predominantly a-helical and β-sheet structure whereas the renatured protein contains predominantly andom-coil content． The natured protein has also a gauche-gauche-gauche configuration of the C-C-S-S-C-C linkage while the Raman lines indicate thatthe geometric difference existing among the disulphide linkages of renatured protein are not large enough to be detected by Raman spectroscopy． The S- CH_2 and the CH_2-CH_2 bond of the former is trans-gauche configuration whereas the latter is gauche- trans configration. about 30％tyrosine residues are buried inside the molecules and 70％tyrosine residues are located On molecular sur-face in natured protein.