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Rapid detection of nivalenol and deoxynivalenol in wheat using surface plasmon resonance immunoassay |
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| Authors: | Tomoyuki Kadota Yoko Takezawa Osamu Tajima Takashi Nakajima Yoichi Kamata |
| Institution: | a Central Laboratories for Frontier Technology, Center for Food Safety Science, Kirin Holdings Company, Limited, 3 Miyahara, Takasaki, Gunma 370-1295, Japan b United Graduate School of Veterinary Sciences, Gifu University, 1-1, Yanagido, Gifu City, Gifu 501-1193, Japan c Mycotoxin Research Unit, National Center for Agricultural Utilization Research, USDA-ARS, 1815 N. University Street, Peoria, IL 61604, USA d Research Team for Fusarium Head Blight Control, National Agricultural Research Center for Kyushu-Okinawa Region, 2421 Suya, Koshi, Kumamoto 861-1192, Japan e Kobe Institute of Health, 4-6 Minatojima-Nakamachi, Chuo-ku, Kobe 650-0046, Japan f Division of Microbiology, National Institute of Health Sciences, 1-18-1, Kamiyouga, Setagayaku, Tokyo 158-8501, Japan |
| Abstract: | A surface plasmon resonance (SPR) immunoassay using a monoclonal antibody was developed to measure nivalenol (NIV) and deoxynivalenol (DON) contamination in wheat. A highly sensitive and stable DON-immobilized sensor chip was prepared, and an SPR detection procedure was developed. The competitive inhibition assay used a monoclonal antibody that cross-reacts with NIV and DON. The half maximal inhibitory concentration (IC50) values of the SPR assay were 28.8 and 14.9 ng mL−1 for NIV and DON, respectively. The combined responses of NIV and DON in wheat were obtained using a simultaneous detection assay in a one-step cleanup procedure. NIV and DON were separated using a commercial DON-specific immunoaffinity column (IAC) and their responses were obtained using an independent detection assay. Spiked tests using these toxins revealed that recoveries were in the range 91.5-107% with good relative standard deviations (RSDs) (0.40-4.1%) and that detection limits were 0.1 and 0.05 mg kg−1 for NIV and DON, respectively. The independent detection using IAC showed detection limits of 0.2 and 0.1 mg kg−1 for NIV and DON, respectively. SPR analysis results were correlated with those obtained using a conventional LC/MS/MS method for wheat co-contaminated with NIV and DON. These results suggested that the developed SPR assay is a practical method to rapidly screen the NIV and DON co-contamination of wheat and one of a very few immunoassays to detect NIV directly. |
| Keywords: | Mycotoxin Surface plasmon resonance Immunoassay |
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