The effects of alternative stress on the cell membrane deformability of chrysanthemum callus cells

The effects of alternative stress, which was generated through a strong sound field apparatus set up in our lab, on cultured chrysanthemum callus cells were studied. Meanwhile we measured the deformability of chrysanthemum cell membranes and studied the influence of the cytoskeleton after the treatment of colchicine using micropipette aspiration technique. Based on our experimental results, we found that the deformability of cell membrane decreased in stress condition. However, the effect disappeared after the treatment of cytochalasin. Therefore, we thought that the reason on the deformability of cells decreasing was the microfilament rearranging and consequently the cells becoming more rigid under the alternative stress.