Effect of electric field switching on the electrophoretic mobility of single-stranded DNA molecules in polyacrylamide gels |
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| Authors: | E Lai N A Davi L E Hood |
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| Institution: | Division of Biology, California Institute of Technology, Pasadena 91125. |
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| Abstract: | We have examined the effects of pulsed electric fields on the separation of single-stranded DNA molecules in polyacrylamide sequencing gels. Using different electric field pulsing regimens, the mobilities of single-stranded DNA molecules can be retarded or increased as compared to conventional electrophoresis. These results indicated that pulsed field techniques can be applied to gel electrophoresis of small single-stranded DNA molecules. |
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