Scale up and Application of Biosurfactant from <Emphasis Type="Italic">Bacillus subtilis</Emphasis> in Enhanced Oil Recovery |
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Authors: | Hossein Amani Mohammad Reza Mehrnia Mohammad Hossein Sarrafzadeh Manouchehr Haghighi Mohammad Reza Soudi |
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Institution: | (1) Oil and Gas Center of Excellence, School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box 11155-4563, Tehran, Iran;(2) Australian School of Petroleum, University of Adelaide, Adelaide, 5000, Australia;(3) Department of Microbiology, Faculty of Sciences, Alzahra University, Vanak, Tehran, Iran |
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Abstract: | There is a lack of fundamental knowledge about the scale up of biosurfactant production. In order to develop suitable technology
of commercialization, carrying out tests in shake flasks and bioreactors was essential. A reactor with integrated foam collector
was designed for biosurfactant production using Bacillus subtilis isolated from agricultural soil. The yield of biosurfactant on biomass (Y
p/x), biosurfactant on sucrose (Y
p/s), and the volumetric production rate (Y) for shake flask were obtained about 0.45 g g−1, 0.18 g g−1, and 0.03 g l−1 h−1, respectively. The best condition for bioreactor was 300 rpm and 1.5 vvm, giving Y
x/s, Y
p/x, Y
p/s, and Y of 0.42 g g−1, 0.595 g g−1, 0.25 g g−1, and 0.057 g l−1 h−1, respectively. The biosurfactant maximum production, 2.5 g l−1, was reached in 44 h of growth, which was 28% better than the shake flask. The obtained volumetric oxygen transfer coefficient
(K
L
a) values at optimum conditions in the shake flask and the bioreactor were found to be around 0.01 and 0.0117 s−1, respectively. Comparison of K
L
a values at optimum conditions shows that biosurfactant production scaling up from shake flask to bioreactor can be done with
K
L
a as scale up criterion very accurately. Nearly 8% of original oil in place was recovered using this biosurfactant after water
flooding in the sand pack. |
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Keywords: | |
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