In situ sol-gel preparation of porous alumina monoliths for chromatographic separations of adenosine phosphates |
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Authors: | Zajickova Zuzana Rubi Emir Svec Frantisek |
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Institution: | Department of Physical Sciences, Barry University, 11300 NE 2nd Ave, Miami Shores, FL 33161, USA. ZZajickova@mail.barry.edu |
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Abstract: | A method enabling the in situ preparation of porous alumina monoliths within 100 μm i.d. fused silica capillaries has been developed. These monoliths were prepared using the sol-gel process from a mixture consisting of an inorganic aluminum salt, a porogen, an epoxide, and a solvent. We investigated the effects of varying the preparation conditions on the physical characteristics of the monoliths with respect to their potential application in chromatographic separations. The best columns were obtained from a mixture of aluminum chloride hexahydrate, N,N-dimethylformamide, water, ethanol and propylene oxide. Adenosine phosphates were then separated in the optimized column with retention increasing according to number of phosphate functionalities. |
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Keywords: | Alumina Monolith Sol–gel Capillary column chromatography Adenosine phosphate derivatives |
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