| Abstract: | An useful electrochemical sensing approach was developed for epinephrine (EP) detection based on graphene quantum dots (GQDs) and laccase modified glassy carbon electrodes (GC). The miniature GC biosensor was designed and constructed via the immobilization of laccase in an electroactive layer of the electrode coated with carbon nanoparticles. This sensing arrangement utilized the catalytic oxidation of EP to epinephrine quinone. The detection process was based on the oxidation of catecholamine in the presence of the enzyme – laccase. With the optimized conditions, the analytical performance demonstrated a high degree of sensitivity ?2.9 μA mM?1 cm?2, selectivity in a broad linear range (1–120×10?6 M) with detection limit of 83 nM. Moreover, the method was successfully applied for EP determination in labeled pharmacological samples. |
