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| 人胶质瘤细胞SWO-3 8神经营养活性物的初步分离 | |
| 引用本文： | 廖异平,郭畹华,顾熊飞.人胶质瘤细胞SWO-3 8神经营养活性物的初步分离[J].宁波大学学报(理工版),2000,13(2):96-101. |
| 作者姓名： | 廖异平 郭畹华 顾熊飞 |
| 作者单位： | 1. 宁波大学医学院，浙江 宁波 315211 2. 中山医科大学组织学与胚胎学教研室神经科学研究室 3. 中山医科大学生物化学教研室，广东 广州 510080 |
| 摘 要： | 制备了人胶质瘤细胞ＳＷＯ－３８条件培养基（ＳＷＯ－３８ＣＭ），经超滤浓缩，截留分子量大于１０ｋＤ的ＳＷＯ－３８ＣＭ，并运用快速自动比色微量分析法检测其对体外培养的ＳＤ大鼠小脑皮质神经元的作用，结果表明，分子量大于１０ｋＤ的ＳＷＯ－３８ＣＭ对神经元的作用，与对照组比较有明显的神经营养活性，分子量大于１０ｋＤ的ＳＷＯ－３８ＣＭ经ＳｅｐｈａｃｒｙｌＳ－２００－ＨＲ凝胶层析并结合生物活性鉴定，获得了具有神 |
| 关 键 词： | 神经营养活性 人胶质瘤细胞 SWO－38条件培养基 |
| 文章编号： | 1001-51 32(2000)02-0096-06 |
Primary Isolation of Human Glioma Cell Line (SWO-38)-derived Neurotrophic Factor |
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| LIAO Yi-ping,GUO Wan-hua,GU Xiong-fei.Primary Isolation of Human Glioma Cell Line (SWO-38)-derived Neurotrophic Factor[J].Journal of Ningbo University(Natural Science and Engineering Edition),2000,13(2):96-101. | |
| Authors: | LIAO Yi-ping GUO Wan-hua GU Xiong-fei |
| Abstract: | Human glioma cell line (SWO-38) conditioned medium (SWO-38 CM)was prepared,ultratiltrated and concentrated, so the fraction of molecular weight (MW)>10 kD from SWO-38CM was obtained. The effect of SWO-38 CM fractions (MW>10 kD and MW<10kD)on theactivity of cultured cerebellar cortical neurons of Spraque-Dawley rat was measured by a rapidautomated colorimetric microassay, respectively. The data indicated that SWO-38 CM fraction(MW>10 kD) possessed significant neurotrophic activity to the neurons, as compared to thecontrol (P<0.05). Additionally, the active fraction of SWO-38 CM was purified further bySephacryl S-200-HR gel chromatography and peak Ⅱ was only identified to possess neurotrophicactivity. |
| Keywords: | neurotrophic activity human glioma cell line conditioned medium cerebellar conical neurons automated colorimetric microassay Sephacryl S-200-HR gel chromatography |
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