Nutritional requirements of a strain of bacillus thuringiensis subsp. kurstaki and use of gruel hydrolysate for the formulation of a new medium for δ-endotoxin production |
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Authors: | N Zouari A Dhouib R Ellouz S Jaoua |
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Institution: | (1) Centre de Biotechnologie de Sfax, BP “358”, 3018 Sfax, TUNISIA |
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Abstract: | Nutritional requirements of a strain ofBacillus thuringiensis (Bt) subsp.kurstaki were elucidated for δ-endotoxin production. The effect of some principal nutrients was deeply investigated, showing several
nutritional and metabolite limitations in Bt growth and δ-endotoxin synthesis. This led us to formulate a new medium based
on the hydrolysate of gruel, a cheap and abundant byproduct of semolina factories, supporting growth and δ-endotoxin synthesis.
After hydrolysis of gruel by α-amylase, followed by proteolysis using alcalase, the resultant soluble material substituted
glucose very well for Bt δ-endotoxin production. Indeed, 15 g/L total sugars coming from that hydrolysate, supplemented by
5.4 g/L ammonium sulfate as nitrogen source and either 5 g/L yeast extract or 3 g/L peptone from casein or 3 g/L casaminoacids
or 0.25 g/L cysteine or aspartic acid, were the principal components of this new medium in which almost 1 g/L of δ-endotoxin
in 4.5 g/L total dry biomass was produced. |
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Keywords: | Bacillus thuringiensis δ -endotoxin gruel hydrolysate metabolite repression optimization of bioinsecticide production |
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