Study on an on-line molecularly imprinted solid-phase extraction coupled to high-performance liquid chromatography for separation and determination of trace estrone in environment |
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Authors: | Zhixiang Xu Shuang Chen Wei Huang Guozhen Fang Hua Pingzhu and Shuo Wang |
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Institution: | (1) Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, 300457, China;(2) College of Food Science and Engineering, Shandong Agricultural University, Taian, 271018, China |
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Abstract: | Estrone is one of the important potential endocrine-disrupting compounds, and the sensitive and reliable analytical methods
for the determination of estrone are required for the assurance of human health. In this paper, using estrone as template
molecule, 3-aminopropyltriethoxysilane as function monomer, and tetraethoxysilicane as cross-linker, a highly selective molecularly
imprinted microsphere was synthesized by surface molecular imprinting technique combined with a sol–gel process. The imprinted
material was characterized by the Fourier transform infrared and static adsorption experiments, and the results showed that
it exhibited good recognition and selective ability for estrone. A novel method for separation and determination of trace
estrone in environmental sample was developed using on-line molecularly imprinted solid-phase extraction coupled to high-performance
liquid chromatography. With a sample loading flow rate of 2.6 mL min−1 for a 9.6-min extraction, the enrichment factor obtained by the slopes of the linear portion in comparison with the direct
injection of 10 μL standard sample solution was 1,045. The detection limit (S/N = 3) was 5.7 ng L−1, and the relative standard deviations for nine replicate extractions of 5.0 μg L−1 estrone was less than 10.0%. This method was evaluated for quantitative determination of estrone in well and lake water samples
spiked at two levels (0.5 and 1.0 μg L−1) with recoveries ranging from 86% to 95%.
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Keywords: | Estrone Molecular imprinting Sol– gel On-line solid-phase extraction High-performance liquid chromatography |
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