| 三种细胞质雄性不育水稻小孢子发育过程的研究 |
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| 引用本文： | 孙天恩,叶梦炜,王明全,陈克成.三种细胞质雄性不育水稻小孢子发育过程的研究[J].武汉大学学报(理学版),1996(6). |
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| 作者姓名： | 孙天恩 叶梦炜 王明全 陈克成 |
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| 作者单位： | 武汉大学分析测试科学系，武汉大学生命科学学院 |
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| 摘 要： | 运用ＤＮＡ荧光探针结合显微荧光术，系统地研究了３种水稻细胞质雄性不育系的败育过程、时期和特点．结果表明，珍汕９７不育系不能完成小孢子第一次有丝分裂；马协不育系小孢子可完成第一次有丝分裂，但不能进入第二次有丝分裂；广丛４１不育系部分小孢子可完成第二次有丝分裂，因此可看到许多三核花粉，但因核行为异常而败育．小孢子的败育过程可能是一种程序性细胞死亡（ＰＣＤ）的过程
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| 关 键 词： | 水稻雄性不育，小孢子，PCD，显微荧光术 |
STUDY ON DEVELOPMENT PROCESS OF MICROSPORE IN THREE MALE STERILE RICE |
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| Sun Tianen,Ye Mengwei,Wang Mingquan,Chen Kecheng.STUDY ON DEVELOPMENT PROCESS OF MICROSPORE IN THREE MALE STERILE RICE[J].JOurnal of Wuhan University:Natural Science Edition,1996(6). |
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| Authors: | Sun Tianen Ye Mengwei Wang Mingquan Chen Kecheng |
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| Abstract: | The process of microspore abortion and its period and chara cteristic in three cytoplasmatic male sterility rice were studied by means of DNA fluoresent probe with microfluorometry. The result showed that ZS97Acouldn't complete the first mitosis of microspore.MAX microspore couldcomplete the first mitosis,but MAX microspore couldn't carry the secondmitosis out; Part of microspore in GC41A could complete the second mitosis,so many trinuclear pollen could be observed in GC41A, but it led to abortionas result of unusual nuclear behavior. We thought that abortion process ofmicrospore was possible a process of PCD. |
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| Keywords: | rice male sterility microspore PCD microfluorometry |
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