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Mapping Receptor Density on Live Cells by Using Fluorescence Correlation Spectroscopy |
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| Authors: | Yan Chen Alina C Munteanu Dr Yu‐Fen Huang Dr Joseph Phillips Dr Zhi Zhu Michael Mavros Weihong Tan Prof |
| Institution: | 1. Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center and Center for Research at Bio/nano Interface, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611 (USA), Fax: (+1)?352‐846‐2410;2. Y.C. and A.C.M. are co‐first authors for this paper. |
| Abstract: | Sensitive surveying : Fluorescence correlation spectroscopy (FCS) was used to study receptor density and to monitor ligand–receptor interactions on live cell membranes by the introduction of fluorescently marked aptamers, which specifically bind to certain cell‐surface receptors (see schematic representation; FITC: fluorescein isothiocyanate).  |
| Keywords: | aptamers fluorescence spectroscopy ligand– receptor interactions receptors |