DNA Binding and Cleavage Activity of Zinc(II) Complex of N,N′‐Bis(2‐guanidinoethyl)‐2,6‐pyridinedicarboxamide |
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| Authors: | Ying Shao Yong Ding Weihong Xu Li Wei Fang Liu Guoyuan Lu |
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| Institution: | 1. State Key Laboratory of Coordination Chemistry, Department of Chemistry, Nanjing University, Nanjing, Jiangsu 210093, China;2. Fax: 0086‐025‐83317761;3. Tel.: 0086‐025‐83685613 |
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| Abstract: | The interaction of zinc(II) complex of N,N′‐bis(guanidinoethyl)‐2,6‐pyridinedicarboxamide (Gua) with DNA was studied by CD spectroscopy and agarose gel electrophoresis analysis. The results indicate that the DNA binding affinity of Zn2+‐Gua is stronger than that of Gua and the Zn2+‐Gua can promote the cleavage of phosphodiester bond of supercoiled DNA under a physiological condition, which is ～106 times higher than DNA natural degradation. The hydrolysis pathway was proposed as the possible mechanism for DNA cleavage promoted by the Zn2+‐ Gua. The acceleration is due to cooperative catalysis of the zinc cation center and the functional groups (bisguanidinium groups). |
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| Keywords: | DNA binding DNA cleavage phosphodiester bond guanidinium group artificial nuclease |
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