| MTT法测定稀土离子对BEL-7402及K562细胞的毒性及增殖毒性 |
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| 引用本文： | 红枫,黄沛力,王夔,李荣昌.MTT法测定稀土离子对BEL-7402及K562细胞的毒性及增殖毒性[J].中国稀土学报,2003,21(5):597-599. |
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| 作者姓名： | 红枫 黄沛力 王夔 李荣昌 |
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| 作者单位： | 1. 北京大学医学部天然药物与仿生药物国家重点实验室,北京,100083
2. 首都医科大学卫生化学教研室,北京,100054 |
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| 基金项目： | 国家自然科学基金重大资助项目(29890280) |
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| 摘 要： | 用MTT法测定稀土离子在不同浓度、不同培养液中,与BEL 7402和K562细胞作用不同时间,对细胞的毒性和增殖毒性。结果表明,在含10%小牛血清培养液中,仅个别稀土离子在较高浓度时对BEL 7402细胞增殖有较弱的抑制作用;对于K562细胞,稀土离子在低浓度时对细胞增殖即表现出较强的抑制作用(P<0.05)。当培养液不含小牛血清时,较低浓度的稀土离子即可抑制BEL 7402细胞的增殖(P<0 05)。
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| 关 键 词： | 细胞生物学 癌细胞 白血病细胞 肝癌细胞 MTT法 稀土 |
| 文章编号： | 1000-4343(2003)05-0597-03 |
| 修稿时间： | 2001年11月12 |
Effects of Rare Earths on Proliferation Toxicity of K562 and BEL-7402 Cells |
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| Hong Feng,Huang Peili,Wang Kui,Li Rongchang.Effects of Rare Earths on Proliferation Toxicity of K562 and BEL-7402 Cells[J].Journal of the Chinese Rare Earth Society,2003,21(5):597-599. |
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| Authors: | Hong Feng Huang Peili Wang Kui Li Rongchang |
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| Institution: | Hong Feng~1,Huang Peili~2*,Wang Kui~1,Li Rongchang~1 |
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| Abstract: | By means of MTT, effects of Ln on the proliferation toxicity of K562 and BEL-7402 cells were studied.The results show that Ln has different proliferation toxicity on the two cancer cell lines in the medium of RPMI1640 containing 10%FCS. Ln inhibits the proliferation of K562 cell strongly at the low concentration, whereas few Ln inhibits the proliferation of BEL-7402 cell slightly only at the high concentration. In the medium of RPMI1640 without 10%FCS Ln inhibits the proliferation of BEL-7402 cell at the low concentration. |
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| Keywords: | cytobiology cancer cell human erythroleukemia cell line K562 human liver cancer cell BEL-7402 3(4 5-dimethylthiazol-2-yl)2 5-diphenyl-tetrazolium bromide rare earths |
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