| 细胞内不同形态Gd基MRI造影剂的T1/T2弛豫性能及造影效果 |
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| 引用本文： | 孙兴丽,张艳辉,赵敏敏,谭波,张海禄,邓宗武.细胞内不同形态Gd基MRI造影剂的T1/T2弛豫性能及造影效果[J].无机化学学报,2019,35(5):780-786. |
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| 作者姓名： | 孙兴丽 张艳辉 赵敏敏 谭波 张海禄 邓宗武 |
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| 作者单位： | 中国科学技术大学苏州纳米技术与纳米仿生学院;中国科学院苏州纳米技术与纳米仿生研究所 |
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| 基金项目： | 国家自然科学基金（No.21673281、31371010），中国科学院青年创新促进会（No.2012242）和中国科学技术部重点研究项目（No.2017YFA0104300）资助 |
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| 摘 要： | 将具有良好生物膜穿透性的异烟肼(INH)和Gd-DO3A偶联,合成了小分子MRI造影剂Gd-DO3A-INH;利用脉冲电转染技术标记间充质干细胞,有效提高了进入细胞的Gd-DO3A-INH浓度,并诱导部分游离态Gd-DO3A-INH在细胞质中自组装成纳米粒子。细胞样品的TEM观察到细胞内形成了Gd-DO3A-INH纳米粒子;细胞传代实验和体外MRI揭示了2种不同状态的Gd-DO3A-INH对细胞水质子弛豫速率的影响机制,以及细胞传代过程中细胞内2种不同状态Gd-DO3A-INH的浓度涨落引起的MRI造影效果的变化机制。
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| 关 键 词： | 磁共振成像(MRI) Gd基造影剂 弛豫速率 脉冲电转染标记 |
| 收稿时间： | 2018/9/20 0:00:00 |
| 修稿时间： | 2019/3/8 0:00:00 |
T1/T2 Relaxation and MRI Contrast Effect of Gd Agent in Different Intracellular Forms |
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| SUN Xing-Li,ZHANG Yan-Hui,ZHAO Min-Min,TAN Bo,ZHANG Hai-Lu and DENG Zong-Wu.T1/T2 Relaxation and MRI Contrast Effect of Gd Agent in Different Intracellular Forms[J].Chinese Journal of Inorganic Chemistry,2019,35(5):780-786. |
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| Authors: | SUN Xing-Li ZHANG Yan-Hui ZHAO Min-Min TAN Bo ZHANG Hai-Lu and DENG Zong-Wu |
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| Institution: | School of Nano Technology and Nano Bionics, University of Science and Technology of China, Hefei 230026, China;Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, Jiangsu 215123, China,Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, Jiangsu 215123, China,Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, Jiangsu 215123, China,Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, Jiangsu 215123, China,Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, Jiangsu 215123, China and Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, Suzhou, Jiangsu 215123, China |
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| Abstract: | Isoniazid (INH) was coupled with Gd-DO3A to yield Gd-DO3A-INH as a small molecule MRI contrast agent with good membrane penetration activity. Pulsed electroporation was used to introduce Gd-DO3A-INH into the cytosol of human mesenchymal stem cells in two different forms:free Gd-DO3A-INH and Gd-DO3A-INH nanoclusters. Cellular TEM demonstrated the formation of intracellular Gd-DO3A-INH nanoclusters. Cell proliferation and cellular MRI demonstrated that the two different forms of Gd-DO3A-INH exerted different effect on the longitudinal (T1) and transverse (T2) relaxations of cellular water protons. Free Gd-DO3A-INH is in favor of acceleration of T1 relaxation, and exhibits fast exocytosis. Gd-DO3A-INH nanocluster is in favor of acceleration of T2 relaxation, and exhibits a promoted cell retention time. As a result, cellular MRI exhibits a fast recovery of T1 relaxation and T1-weighted signal intensity/contrast, and a delayed recovery of T2 relaxation and T2-weighted signal intensity/contrast during cell proliferation. |
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| Keywords: | magnetic resonance imaging (MRI) Gd contrast agent magnetic relaxivity pulsed electroporation |
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