pH调控Y型分子筛负载阿霉素纳米药物制备及与MM-231细胞的作用

针对抗肿瘤小分子药物靶向性差、疗效低和毒副性大等缺陷,我们以Y型分子筛(YMS)为基体、阿霉素(DOX)为药物模型,通过pH调控,借助氢键和范德华力等物理作用力制备得到高负载Y型分子筛纳米药物体系(YMS?DOX)。采用UV?Vis、FT?IR、粒径和电位测试及荧光光谱证实YMS?DOX成功制备,且DOX的负载率可高达99.61%。体外药物释放测试发现YMS?DOX具有pH响应释放特性,在肿瘤环境中(pH=4.5)的药物释放量为正常生理环境(pH=7.4)中的3.8倍,表明其具有良好的药物输送特性。此外,利用流式细胞术和MTT测试法探究了YMS?DOX对乳腺癌细胞(MM?231)和树突细胞(DC)的细胞凋亡和毒性,结果表明YMS?DOX可以诱导肿瘤细胞凋亡,且可降低对正常细胞的毒副作用。

pH Regulated Nanomedicine Based on Y-Type Molecular Sieve Loading Doxorubicin: Preparation and Interaction with MM-231 Cells

To overcome the shortcomings of poor antitumor drug targeting, low efficacy, and high toxicity, a pH regulated drug delivery system (YMS-DOX) through hydrogen bonds and van der Waals force was constructed to acquire high drug loading capacity and better therapeutic efficiency based on Y-type molecular sieve (YMS) as nano-carrier and doxorubicin (DOX) as the model drug. The successful formation of YMS-DOX was confirmed by using UV-Vis, FT-IR, particle size and potential measurement, and fluorescence spectroscopy. Interestingly, with the assistance of citric acid buffer solution (pH=9.0), YMS nanocarrier loading DOX achieved nearly 99.61% loading efficiency. In vitro drug release showed that YMS had low premature drug release under physiological conditions (pH=7.4), while greatly enhanced in the tumor microenvironment (pH=4.5) 3.8 times compared to normal tissue, indicating YMSDOX can be effectively released in the tumor site. Excitingly, when dendritic (DC) cells and breast cancer (MM -231) cells were treated with YMS-DOX, the results demonstrated that YMS-DOX preferentially accumulated much more in tumor cells than in normal cells, which implies that YMS-DOX can selectively kill tumor cells. In addition, assessment by flow cytometry apoptosis assay illustrated that YMS-DOX could induce cell apoptosis and inhibit cell migration.