适配体传感器检测凝血酶的研究进展

适配体是通过指数富集配体系统进化技术(SELEX)筛选得到的,能与多种目标物质高特异性、高选择性结合的寡核苷酸序列.适配体因合成简单、稳定性高、设计多样化、修饰方便、成本低等优点被用作为识别探针,并与不同的转导技术相结合,构建了多种类型的适配体传感器,并借助纳米材料独特的光、电特性,提高适配体传感器的性能.凝血酶是一种...

Advances in the detection of thrombin by aptamer sensors

Aptamers are oligonucleotide sequences selected by exponential enrichment ligand system evolution techniques(SELEX),which can bind to multiple target substances with high specificity and selectivity.Aptamers have been used as recognition probes because of their advantages of simple synthesis,high stability,diverse design,convenient modification and low cost.In combination with different transduction techniques,various types of aptamer sensors have been constructed,and the performance of aptamer sensors has been improved with the help of the unique optical and electrical properties of nanomaterials.Thrombin is a multifunctional serine protease,which is a major stimulant for procoagulant and anticoagulant and plays an important role in vascular injury and various pathological activities such as,thrombotic diseases,atherosclerosis and tumors.Therefore,it is necessary to develop effective methods for highly selective and specific detection of thrombin.The application of aptamer sensors based on fluorescence,colorimetric,electrochemiluminescence,electrochemical,surface enhanced Raman scattering and nucleic acid amplification in thrombin detection is reviewed.