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高效液相色谱法同时测定强化牛奶中的维生素A和D_2
引用本文:赵慧芬,李克杰,曹燕叔,郑建国. 高效液相色谱法同时测定强化牛奶中的维生素A和D_2[J]. 色谱, 1995, 13(1): 61-62
作者姓名:赵慧芬  李克杰  曹燕叔  郑建国
作者单位:北京奶牛中心
摘    要:
A reversed-phase high performance liquid chromatographic method for the simultaneous determination ofvitamin A and D_2 in fortified milk has been developed. A couple of serially connected columns with μ-Bonda-pak C_(18)(300 × 3. 9mm, 10μm)and Novapak C_(18)(150 × 3. 9mm, 4μm)and methanol/water(96/4)mobile phasewere used. Vitamin A and D_2 were detected at wavelengths of 325nm and 265nm, respectively. By usingsaponification procedure, the recoveries of vitamin A and D_2 were in the range of 90~110%.

关 键 词:高效液相色谱  强化牛奶  维生素A和D_2  

Simultaneous Determination of Vitamin A and D_2in Fortified Milk by High Performance Liquid Chromatography
Zhao Huifen,Li Kejie ,Cao Yanshu and Zheng Jianguo. Simultaneous Determination of Vitamin A and D_2in Fortified Milk by High Performance Liquid Chromatography[J]. Chinese journal of chromatography, 1995, 13(1): 61-62
Authors:Zhao Huifen  Li Kejie   Cao Yanshu  Zheng Jianguo
Abstract:
Keywords:high performance liquid chromatography  vitamin A and D_2  fortified milk
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