| 聚天冬氨酸硅质固定相的研制及应用 |
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| 引用本文： | 刘淑娟,祝馨怡,周文峰,刘霞,蒋生祥. 聚天冬氨酸硅质固定相的研制及应用[J]. 分析测试技术与仪器, 2003, 9(2): 80-83 |
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| 作者姓名： | 刘淑娟 祝馨怡 周文峰 刘霞 蒋生祥 |
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| 作者单位： | 中国科学院兰州化学物理研究所,甘肃,兰州,730000;中国科学院兰州化学物理研究所,甘肃,兰州,730000;中国科学院兰州化学物理研究所,甘肃,兰州,730000;中国科学院兰州化学物理研究所,甘肃,兰州,730000;中国科学院兰州化学物理研究所,甘肃,兰州,730000 |
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| 摘 要： | D,L-天冬氨酸在浓磷酸的存在下加热聚合生成聚琥珀酰亚胺,此产物能与氨丙基硅胶快速反应生成聚琥珀酰胺硅胶,然后再水解生成聚天冬氨酸硅质固定相．并对反应条件进行了优化、实验表明,聚天冬氨酸固定相对蛋白质有较好的分离能力和选择性．
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| 关 键 词： | HPLC D L-天冬氨酸 固定相 蛋白质 |
| 文章编号： | 1006-3757(2003)02-0080-04 |
| 收稿时间： | 2003-03-05 |
| 修稿时间： | 2003-03-05 |
Synthesis and Application of Poly(D, L-aspartic Acid)-Silica Stationary Phase |
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| LIU Shu-juan,ZHU Xin-yi,ZHOU Wen-feng,LIU Xia and JIANG Sheng-xiang. Synthesis and Application of Poly(D, L-aspartic Acid)-Silica Stationary Phase[J]. Analysis and Testing Technology and Instruments, 2003, 9(2): 80-83 |
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| Authors: | LIU Shu-juan ZHU Xin-yi ZHOU Wen-feng LIU Xia JIANG Sheng-xiang |
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| Affiliation: | Lanzhou Institute of Chemical Physics, CAS, Lanzhou 730000, China;Lanzhou Institute of Chemical Physics, CAS, Lanzhou 730000, China;Lanzhou Institute of Chemical Physics, CAS, Lanzhou 730000, China;Lanzhou Institute of Chemical Physics, CAS, Lanzhou 730000, China;Lanzhou Institute of Chemical Physics, CAS, Lanzhou 730000, China |
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| Abstract: | In the presence of concentrate H3PO4 the poly(succinimide) was quantitatively polymerized by heating D,L-aspartic acid. The product quickly reacted with aminopropyl-silica, and then was hydrolyzed to poly(aspartic acid)-silica. It was found that the poly(aspartic acid)-silica stationary phase had good ability to separate proteins. The reaction conditions were also optimized during the investigation. |
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| Keywords: | HPLC D L-aspartic acid stationary phase protein |
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