Wetting of phospholipid membranes on hydrophilic surfaces - Concepts towards self-healing membranes |
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Authors: | J Nissen S Gritsch G Wiegand JO Rädler |
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Institution: | Institut für Biophysik (E22), Physik Department, Technische Universit?t München, James Franck Stra?e 1, 85747, Garching, Germany
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Abstract: | We report on the wetting behavior of phospholipid membranes on solid surfaces immersed in aqueous solution. Using fluorescence
microscopy, the spreading velocity of fluid bilayers advancing from a lipid source is investigated. The kinetic spreading
coefficient was measured as a function of temperature for pure DMPC membranes and as a function of charge density and cholesterol
content for binary membranes. A theoretical model for the membrane flow is presented, which takes into account the liquid
crystalline bilayer architecture of the lipid membrane. The spreading power results from the membrane-solid VdW interaction
and is dissipated in hydrodynamic shear flow as well as by inter-monolayer friction within the bilayer. The frictional drag
causes a dynamic tension gradient in the spreading membrane, which is manifested by a single exponential decay of the fluorescence
intensity profile along the spreading direction. Obstacles are shown to act as pinning centers deforming the advancing line
interface. However, no depinning was observed, since the centers are circumflown without abrupt relaxation.
Received 6 November 1998 |
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Keywords: | PACS 68 10 Gw Interface activity spreading - 68 45 Gd Wetting - 87 16 Dg Membranes bilayers and vesicles |
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