Fluorescence of proteins induced by two-photon absorption |
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| Authors: | Y W Xu J R Zhang Y M Deng L K Hui S P Jiang S H Lian |
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| Affiliation: | Anhui Institute of Optics and Fine Mechanics, Academia Sinica, Hefei, China. |
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| Abstract: | At room temperature, the UV fluorescence of trypsin, thyroglobulin, hemoglobin and albumin are observed; these molecules undergo two-photon absorption when they are excited with the radiation of a frequency-duplicated Q-switched Nd:YAG laser. It is shown that the fluorescence of these proteins comes from the tryptophan residues and that it has a red shift of about 20 nm relative to the one-photon fluorescence. We suggest that the weak emission from tyrosine arises from the forbidden two-photon transition. The influence of concentration on the fluorescence of hemoglobin and tryptophan is discussed. The two-photon absorption cross-sections are estimated. |
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