| 反相高效液相色谱法同时测定沙棘叶中绿原酸和芦丁 |
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| 引用本文： | 赵二劳,王迎进,薛丽,刘越. 反相高效液相色谱法同时测定沙棘叶中绿原酸和芦丁[J]. 分析科学学报, 2012, 28(1): 101-103 |
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| 作者姓名： | 赵二劳 王迎进 薛丽 刘越 |
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| 作者单位： | 1.忻州师范学院化学系,山西忻州,034000;2.忻州师范学院化学系,山西忻州,034000;3.忻州师范学院化学系,山西忻州,034000;4.忻州师范学院化学系,山西忻州,034000 |
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| 基金项目： | 山西省高校科技开发研究项目 |
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| 摘 要： | 建立了同时测定沙棘叶中绿原酸和芦丁含量的反相高效液相色谱(RP-HPLC)法.采用Kromasil C18色谱柱(150×4.6mm,5μm),流动相为体积比20∶80的乙腈-1％乙酸水溶液,检测波长358 nm.结果表明:绿原酸、芦丁的浓度分别在0.0284～0.1704mg·mL-1(r =0.9995)和0.0566～0.3396mg·mL-1(r =0.9992)范围内与色谱峰面积呈良好线性关系;平均加标回收率分别为100.5％(n=5)和98.5％(n=5).该方法快速、准确,重现性好,可用于同时测定沙棘叶中绿原酸和芦丁含量.
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| 关 键 词： | 沙棘叶 绿原酸 芦丁 反相高效液相色谱法 |
Determination of Chlorogenic Acid and Rutin Contents in Seabuckthorn Leaves by RP-HPLC |
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| ZHAO Er-lao , WANG Ying-jin , XUE Li , LIU Yue. Determination of Chlorogenic Acid and Rutin Contents in Seabuckthorn Leaves by RP-HPLC[J]. Journal of Analytical Science, 2012, 28(1): 101-103 |
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| Authors: | ZHAO Er-lao WANG Ying-jin XUE Li LIU Yue |
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| Affiliation: | (Department of Chemistry,Xinzhou Teachers University,Xinzhou 034000) |
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| Abstract: | A RP-HPLC method for simultaneous determination of chlorogenic acid and rutin in Seabuckthorn leaves was established.A Kromasil C18 column(150×4.6 mm,5 μm) was used with acetonitrile-1% acetic acid(20∶80) as the mobile phase,and UV-detection wavelength was set at 358 nm.The linear range was 0.0284 ～0.1704 mg·mL-1(r =0.9995) and 0.0566～0.3396 mg·mL-1(r=0.9992) for chlorogenic acid and rutin,respectively.The average recoveries(n=5) were 100.5% and 98.5%,respectively.This method is rapid,accurate with good reproducibility,which can be applied to the determination of chlorogenic acid and rutin in Seabuckthorn leaves. |
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| Keywords: | Seabuckthorn leaves Chlorogenic acid Rutin Reversed phase high performance liquid chromatography |
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