| Abstract: | Abstract A simple, sensitive, and specific flow injection fluorimetric method has been developed for the determination of carbamazepine (CBZ). The proposed method is based on use of a solid‐phase reactor containing lead dioxide for on‐line oxidization of CBZ into a strongly fluorescent compound in a medium of phosphoric acid. The product has a green‐yellow fluorescence at a maximum excitation wavelength of 355 nm and an emission wavelength of 478 nm. Under the optimum conditions, the fluorescence intensity is proportional to the concentration of CBZ ranging from 0.0005 to 4.000 µg mL?1. The detection limit is 5.7×10?5 µg mL?1 (2.4×10?10 mol L?1) and the relative standard deviation is 1.4% at the sampling rate of 45 h?1. The proposed method has been applied to clinical estimation of CBZ in real patients' plasma samples with the results compared with those obtained by HPLC method. |
