| Abstract: | Abstract Two methods have been developed for the simultaneous determination of Amitryptiline and Perphenazine: the zero-crossing (I) and the derivative ratio spectrum (II) methods and both use the derivative spectrophotometry. The methods have been applicable in the ranges of 1 to 30 μg·ml?1 of Amitryptiline (I and II) and between 1 and 8 μg·ml?1 and from 1 to 7 μg·ml?1 of Perphenazine for the methods I and II respectively. The accuracy of the proposed methods have been studied and they have been used in the determination of Amitryptiline and Perphenazine in commercially available pharmaceuticals. |
