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Determination of Paraquat by Cucurbit[7]uril Sensitized Fluorescence Quenching Method
Abstract:A method for the determination of paraquat by cucurbit7]uril (CB7]) fluorescence quenching was developed. The assay was based on the reaction of the CB7] with acridine orange. The fluorescence intensity of acridine orange regularly increased with the addition of CB7]. However, while an appropriate amount of paraquat was added to the CB7]- acridine orange system, the fluorescence intensity of the system was quenched which was employed to determine paraquat. Under the optimum conditions, a linear range of 3.0–800 nmol L?1 and a detection limit of 1.61 nmol L?1 for paraquat were obtained. The simple strategy reported here offers great practical potential for the determination of pesticide residues in agricultural products.
Keywords:Competitive inclusion  Cucurbit[7]uril  Fluorescence spectroscopy  Paraquat
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