Chiral Capillary Electrophoresis with Highly Sulfated Cyclodextrins; Resolution of Benzoxazolinone Aminoalcohols,and Aminoketon Precursors,Potential Adrenergic Ligands

Today, capillary zone electrophoresis (CZE) is became an established method for the determination of chiral impurities. A method for the enantioresolution of these benzoxazolinone aminoalcohols and their aminoketon precursors, potential adrenergic ligands, was developed using neutral and anionic cyclodextrins as chiral selectors. Operational parameters, such as the nature and concentration of the chiral selectors, temperature, and applied voltage, were investigated. The influence of the structural features of the solutes on migration time and resolution was studied. The apparent and averaged binding constants of complexes were calculated.