| Abstract: | ABSTRACT High sensitive glucose biosensors were realised by oxidative polymerisation of amphiphilic pyrrole monomer-glucose oxidase mixtures, previously adsorbed on platinum electrodes. These sensors, based on H2O2 electrooxidation at 0.5V vs SCE, exhibited marked interferences due to electrooxidisable endogenous (ascorbate and urate) and exogenous (paracetamol) compounds. Bilayer structures, combining the preceding polymer film as an outer layer and electrogenerated poly(phenylene diamine), overoxidised polypyrrolic films or Nafion as an inner layer, were fabricated in order to minimise interferences. Finally, the use of Nafion as a semipermeable barrier appeared to be more efficient than the electrogenerated films. The Nafion-based biosensor exhibited glucose sensitivity of 0.4 mA.M?1; .cm?2, while interference of ascorbate, urate and paracetamol was negligible. |
