Fractionation of lyophilized MIBI kit for99mTc labeling

Summary The lyophilized MIBI kit was dissolved in 1 ml sterile saline or 250<span style='font-size: 12.0pt;font-family:Symbol;mso-bidi-font-family:Symbol'>mg/ml ascorbic acid and dispensed into 0.2 ml fractions, which were stored at -20 °C for 12 days. The solution was prepared by using two different protection methods. In the first method evacuated vials were used for storage of fractionated solution while in the second method an antioxidant agent, ascorbic acid was employed. The radiochemical impurity of ^99mTc-MIBI in the unprotected fractions rises with time. Exclusion of air as well addition of ascorbic acid in fractionated solutions gave very good results. The labeling efficiency and biodistribution of fractionated solutions was the same as the lyophilized kit even after 12 days.