Analysis of Nitrofuran Residues in Animal Feed Using Liquid Chromatography and Photodiode-Array Detection |
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Authors: | P Viñas N Campillo L Carrasco M Hernández-Córdoba |
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Institution: | (1) Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia, Spain |
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Abstract: | The analysis of four nitrofuran veterinary drugs, nitrofurantoin, furazolidone, furaltadone and nitrofurazone, was optimized
using reversed-phase liquid chromatography with a monolithic column and photo-diode array detection. The antibiotics were
extracted from animal feeds by heating with acetonitrile. The isocratic mobile phase consisted of a 8:92 (v/v) acetonitrile/phosphate buffer (pH 4.5) at a flow-rate of 1 mL min−1. Peaks were identified by the retention characteristics and UV spectra. Detection limits in the water samples ranged between
0.21 and 0.27 μg L−1, and in the feed samples between 2.1 and 2.7 μg kg−1, depending on the nitrofuran. The procedure was applied to the control of nitrofuran residues in farm water for poultry and
different animal feeds. |
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Keywords: | Column liquid chromatography Nitrofuran in animal feed Nitrofurantoin Furazolidone Furaltadone Nitrofurazone |
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