| 偶氮胂Ⅲ—Ba(Ⅱ)共振散射光谱法测定人血清蛋白 |
| |
| 引用本文： | 赵长容,方芳,连国军,陈露茜,王林洲.偶氮胂Ⅲ—Ba(Ⅱ)共振散射光谱法测定人血清蛋白[J].广东微量元素科学,2010,17(9):29-33. |
| |
| 作者姓名： | 赵长容 方芳 连国军 陈露茜 王林洲 |
| |
| 作者单位： | 温州医学院环境与公共卫生学院,浙江,温州,325035 |
| |
| 基金项目： | 浙江省分析测试基金项目 |
| |
| 摘 要： | 在pH 3.0~3.8的BR缓冲溶液中,偶氮胂Ⅲ-Ba(Ⅱ)与蛋白质结合形成复合物,导致体系的共振散射信号增强,据此建立了蛋白质测定的共振散射光谱法。结果表明,在优化的实验条件下,牛血清白蛋白(BSA)的线性范围为0~1.0 mg/L,检出限为21.4μg/L;将该法用于人血清中蛋白质的测定,与医院测定结果基本一致;方法的批内精密度为2.8%(n=6),批间精密度为3.5%(n=6),回收率为96.0%~100.0%。
|
| 关 键 词： | 蛋白质 共振散射 偶氮胂Ⅲ-Ba（Ⅱ） |
Determination of Protein in Human Serum by Arsenazo Ⅲ-Ba(Ⅱ)Resonance Light Scattering |
| |
| ZHAO Changrong,FANG Fang,LIAN Guojun,CHEN Luxi,WANG Linzhou.Determination of Protein in Human Serum by Arsenazo Ⅲ-Ba(Ⅱ)Resonance Light Scattering[J].Trace Elements Science,2010,17(9):29-33. |
| |
| Authors: | ZHAO Changrong FANG Fang LIAN Guojun CHEN Luxi WANG Linzhou |
| |
| Institution: | (Department of Environment Science and Public Health,Wenzhou Medical College, Wenzhou 325035,China) |
| |
| Abstract: | In a medium of britton-robinson buffer（pH 3.0~3.8）,a compound was formed between Arsenazo Ⅲ-Ba（Ⅱ）and proteins,which lead to great enhancement of the reonacence light scattering singal of ArsenazoⅢ-Ba（Ⅱ）.Based on this,a novel resonance light scattering method was developed for the determination of proteins in aqueous solution.Under optimal conditions,the linear range of calibration curve for the determination of bovine serum albumin（BSA）was 0~1.0 mg/L,the detection limit was 21.4 μg/L.The method has been applied to the determination of total protein in human serum samples and the results were agreement with those reported by the hospital.The precision was tested at optimum condition.Intra-CV=2.8%（n=6）,inter-CV=3.5%（n=6）and the recovery was in the range of 96.0%~100.0%. |
| |
| Keywords: | protein resonance light scattering arsenazo Ⅲ-Ba（Ⅱ） |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
