SOD-二茂铁修饰电极的电化学行为

SOD(超氧化物歧化酶)广泛存在于自然界一切生物体内，是生物学前沿的研究课题之一，前人对其进行了深入的研究。本文将SOD和二茂铁用新鲜蛋清这种廉价、易得的膜固定剂代替BSA(牛血清白蛋白)修饰到电极上，研究它们在修饰电极上与过氧化氢的作用，并进一步证明SOD与二茂铁之间是否存在着协同效应。

Electrochemical behaviors of superoxide dismutase(SOD)-ferrocene modified gold electrode

Ferrocene and SOD-ferrocene modified gold electrodes were made by crosslinkage method with fress egg white and glutaraldehyde,and cyclic voltammetry was used to study these electrodes in phosphate buffer solution of different pH values and different concentrations of H_2O_2.The results indicated that in alkaline solution the cathodic response of ferroence was inhibited more greatly than the anodic response of ferrocene.The results also indicated that the cathodic peak current of ferrocene decreased rapidly while the anodic peak current decrased slightly with the addition of H_2O_2.But the ferrocene modified on the electrode was not lost,so we concluded that ferroence(+1)could be catalytic to the decomposition of H_2O_2.The behavior of SOD-ferrocene modified electrode indicated that SOD could prohibit the decrease of the peak current of ferrocene in the presence of H_2O_2.But with the increase of concentrations of H_2O_2 the peak currents of SOD-ferrocene modified electrode began to decrease too;and it could take on the same electrohehavior of ferrocene modified electrode when the concentrations of H_2O_2 are high enough.