高效毛细管电泳法同时测定多种肽类药物的方法研究

  以血管紧张素Ⅰ，Ⅱ，Ⅲ和P物质、神经激肽、生 长激素释放的抑制因子、神经紧张肽等7种肽类药物混合物为测定对象，用胺涂层的毛细管 柱(57 cm×75 μm i．d．，有效长度为50 cm)对上述混合物的分离条件进行了研究。在 f工作电压为10 kV［（－）→(＋)］、电流为42 μA、检测波长为214 nm、测定温度为2 5 ℃、虹吸进样10 s的条件下，在50 mmol/L的醋酸铵缓冲液(pH 4.5)中，上述混合物可在8 min内得以完全分离。 关键词：

Study on the Determination of Peptide Mixture by HPCE

A rapid method for the simultaneous assay of 7 peptide mixture, including angiotensin I, II, III, substance P, neurokinin, somatostatin and neurotensin, by high performance capillary electrophoresis has been established. The nature, pH and concentration of buffer, running voltage, detection wavelength, injection time and the effective length of amino-coated capillary were defined with the results of experiment. With 50 mmol/L ammonium acetate (pH 4.5) as running buffer and siphonage injection for 10 seconds, the measurements were carried out at 25 degrees C and 10 kV running voltage (-)-->(+)] applied to a 57 cm x 75 microns i.d. (50 cm effective length) amino-coated capillary. The 7 peptide mixture was determined by a UV detector at 214 nm. The total time for separation and determination was within 8 min. The recoveries ranged from 95% to 98% with RSD from 2.9% to 4.2%. It has been found that the 75 microns i.d. capillary has higher sensitivity than 50 microns, but its efficiency and Rs were worse.