HPLC of amino acids and oligopeptides by pre-column fluorescence derivatization with N-hydroxysuccinimidyl-α-(9-phenanthrene)-acetate |
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| Authors: | J M You G H Xie H E Wang J X Su C L Zhou |
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| Institution: | (1) Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, 730000 Lanzhou, P. R. China;(2) Department of Environmental Engineering, Shandong University, 250100 Jinan, P.R. China;(3) Present address: Department of Chemistry, Jining Medical College Jining, 273 100 Shandong, P. R. China |
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| Abstract: | Summary A sensitive LC method for the detection of amino acids and oligopeptides with pre-column fluorescence derivatization has been
developed. Glycine, glycylglycine, triglycine, glutathione, glutamic acid, and cysteine were separated on a reversed-phase
C18 column with methanol-water-triethylamine eluent, derivatization and chromatographic conditions were optimized. The six derivatives
were eluted in 20 min with good reproducibility. The relative standard derviations (n=6) at an analytical concentration of
2×10−6 M are <5%. Detection limits (signal-to-noise ratio=3) for the six derivatives are 23–68 fmol. |
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| Keywords: | Column liquid chromatography Amino acids and oligopeptides Fluorescence derivatization N-hydroxysuccinimidyl-α -(9-phenanthrene)-acetate |
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