不同晶相草酸钙超细微晶的合成及其与硫酸软骨素A的相互作用

采用配位沉淀法制备了二水草酸钙(COD)和一水草酸钙(COM)超细微晶,其尺寸分别为150nm和320nm。通过扫描电子显微镜(SEM)、X射线衍射(XRD)、红外光谱(FTIR)、纳米粒度仪(Nano-ZS)和紫外-可见吸收光谱(UV-Vis)表征了这两种草酸钙微晶。研究了尿大分子硫酸软骨素A(C4S)对上述COM和COD微晶ξ电位、粒径、聚集程度和紫外吸光度的影响。随着cC4S从0增加到1.0g·L-1,COD微晶的ξ电位从-9.7mV减小到-46.1mV,COM微晶的ξ电位从-15.9mV减小到-49.0mV;微晶表面ξ电位变负后,有利于稳定溶液中悬浮的微晶。在水溶液中,COD和COM微晶均存在显著的聚集现象,而C4S的存在可抑制COD微晶的聚集,并在浓度为0.05g·L-1时抑制效果最好。由于尿液中存在大量草酸钙微晶,本研究有助于阐明草酸钙结石的形成机理和C4S对草酸钙结石形成的抑制作用。

Preparation of Ultrafine COM and COD Crystallites and Their Interaction with Chondroitin Sulfate A

Ultrafine crystallites of calcium oxalate dihydrate (COD) and monohydrate (COM), with a size of 150 nm and 320 nm respectively, were prepared by complexing precipitation method. Scanning electron microscopy (SEM), X-ray diffractometer (XRD), FTIR, nano-particle size analyzer (Nano-ZS) and ultra-visible (UV-Vis) spectrum were used to characterize these two ultrafine crystallites. The effect of urinary macromolecule of chondroitin sulfate A (C4S) on the properties of ultrafine COM and COD crystallites was inv...