On-line calorimetry as a technique for process monitoring and control in biotechnology

This contribution reviews laboratory-scale investigations carried out on the usefulness of biological heat release measurements, as a means for monitoring and controlling the metabolic state of microbial cultures. Such studies are carried out in high-quality bench-scale calorimeters, but measuring heat generation rates by establishing energy balances ought to be applicable to large-scale bioreactors without resorting to sophisticated instrumentation. The signal received can either be interpreted by more qualitative correlation with the evolution of the culture, or it may be quantitatively exploited - together with other on-line measurements - in order to assess the rates at which various types of metabolisms proceed in the culture. The work described shows how this can be used to keep a culture in a desired metabolic state during fed-batch and transient continuous cultures of the yeast, S. cerevisae, and how a bacterial fed-batch culture can be controlled in order to optimize biosynthesis of an antibiotic.