离子相互作用色谱法分离金属离子的研究

离子相互作用色谱在无机离子的分离和分析中潜力很大。它通过离子相互作用试剂,能容易控制或改变柱交换容量或分析物的保留值,分离效率高,可快速完成多组分的同时分离。 在ODS固定相上涂敷阳离子型的十六烷基吡啶(CPI),使金属离子成为阴离子型的氨羧络合物,因不同金属氨羧络合物与CPI的作用程度不同从而可使金属离子分离。金属氨羧络合物亲水性很强,在键合相上的吸附作用可忽略,流动相中不需加入有机溶剂,因此络合物

Separation of Metal Ions by Ion-interaction Chromatography

Some metal ions are separated on a 10 urn bonded column coated with cetylpyridinium by using aminopolycarboxylic acid as the complex reagent. The retention of the metal complexes is due to ion-interaction between the anionic complexes and the sorbed cationic cetylpyridinium. Bi,Co, Pb, Mn, Ni and Cu ions can be completely separated with EDTA as the comoplex reagent,and the detection limits of the metal ions are 0. 8, 2, 30, 100, 80 and 50 ng at 230 nm respectively. Co, Bi, Ni, Fe, Cu and Pb ions can be completely separated with DTPA as the complex reagent, and the detection limits of the metal ions are 80, 4, 400, 5, 20 and 100 ng at 254 nm respectively.