Induction and repair of cyclobuta-dithymidine photoproducts in hamster skin by solar-simulated UV radiation |
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| Authors: | P T Strickland J S Creasey |
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| Affiliation: | Department of Environmental Health Sciences, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205. |
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| Abstract: | The induction and repair of cyclobuta-dithymidine (T less than greater than T) photoproducts in hamster skin was measured following exposure to artificial solar radiation. DNA extracted from irradiated hamster epidermis was analyzed by enzyme-linked immunosorbent assay (ELISA) using a monoclonal antibody specific for T less than greater than T photoproducts. Between 49% and 58% of T less than greater than T antibody binding sites were eliminated from DNA by 24 h after radiation exposures comparable with approximately 60 - 120 min of midday sunlight, whereas all detectable T less than greater than T binding sites were eliminated by 24 h after a radiation exposure comparable with 30 min of sunlight. |
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